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The announcement comes as Nigeria is experiencing its largest outbreak, with 481 confirmed cases reported as of last week, 133 of them fatal.
CEPI, a vaccine development partnership, was created in the wake of Ebola.
The infected Americans are health workers, ages 33 and 47, the latter of whom died.
The man was hospitalized after returning from West Africa and died last night from a Lassa virus infection.
Minnesota health officials diagnosed Lassa fever in a man who had traveled to West Africa.
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